Stock solution stability Calculation

The FDA requires that stock solutions should not be made from reference materials that are about to expire unless the purity of the analyte in the stock solutions is reestablished. When the stock solution exists in a different state (e.g., solution versus solid) or in a different buffer composition (which is generally the case for macromolecules) from the certified reference standard, the sponsor should generate stability data on stock solutions to justify the duration of stock solution storage stability.

High QC

True Concentration Measured Concentration 1 Measured Concentration 2 Measured Concentration 3

Medium QC

True Concentration Measured Concentration 1 Measured Concentration 2 Measured Concentration 3

Low QC

True Concentration Measured Concentration 1 Measured Concentration 2 Measured Concentration 3
QC Level Mean Accuracy Standard Deviation CV (%)
High QC
Medium QC
Low QC