Recovery Calculation

Recovery(%)=Peak area from samples spiked in biological matrix/Peak area from samples spiked in water x 100.

Here you need to spike your drug into biological matrix (e.g., plasma, tissue, urine, feces) or water first, followed by extraction and injection.

Please use the original peak area for your calculation. DONOT use the calculated concentrations

High Concentration

Sample Peak Area in Biological Matrix Peak Area in Water
1
2
3

Medium Concentration

Sample Peak Area in Biological Matrix Peak Area in Water
1
2
3

Low Concentration

Sample Peak Area in Biological Matrix Peak Area in Water
1
2
3
Concentration Mean Recovery (%) Standard Deviation
High
Medium
Low