Matrix Effect Calculation

Matrix Effect(%)=Peak area from samples spiked in matrix from biological blank/Peak area from samples spiked in matrix from water x 100.

Here you need to extract the blank biological matrix (e.g., plasma, tissue, urine, feces) or water first, then spike your drug into the matrix followed by injection.

Please use the original peak area for your calculation. DO NOT use the calculated concentrations.

High Concentration

Sample	Peak Area in Biological Matrix	PeakArea in Water
1
2
3

Medium Concentration

Sample	Peak Area in Biological Matrix	Peak Area in Water
1
2
3

Low Concentration

Sample	Peak Area in Biological Matrix	Peak Area in Water
1
2
3

Concentration	Mean Matrix Effect (%)	Standard Deviation
High
Medium
Low